The Kangaroos edged out England 16-12 in front of 20,585 fans at Melbourne's AAMI Park on Sunday afternoon.
England led Australia 12-4 at half time before the Kangaroos hit back with two second half tries.
Knights utility back Sione Mata'utia became the youngest Kangaroo to debut, while fellow Knight Beau Scott suffered a hamstring injury in the first half.
The Kangaroos now take on Samoa at Wollongong next Sunday.
Australia 16 (Michael Jennings, Ben Hunt, Greg Inglis, Tries; Cameron Smith 2 Goals)
England 12 (Kallum Watkins, Ryan Hall, Tries; Gareth Widdop 2 Goals)
Photo credit: nrlphotos.com